Dispersed Oil disrupts Microbial Pathways in Pelagic Food Webs (2011)
Metadata:
- Identification_Information:
- Citation:
- Citation_Information:
- Originator:
- DISL: Alice Ortmann
- Publication_Date:
- 2012
- Title:
- Dispersed Oil disrupts Microbial Pathways in Pelagic Food Webs (2011)
- Geospatial_Data_Presentation_Form:
- spreadsheet
- Description:
- Abstract:
- Abundances of viruses, prokaryotes, diatoms, dinoflagellates, ciliates and heterotrophic nanoflagellates were determined over time in mesocosm experiments measuring the effects of oil, dispersant and dispersed oil on the microbial loop. Two separate experiments were carried out in June and August 2011. Abundances in the treated mesocosms were compared to a no addition control and a glucose addition control.
- Purpose:
- These experiments were designed to quantify the effects of oil, dispersant and dispersed oil on the coastal pelagic microbial food web.
- Time_Period_of_Content:
- Time_Period_Information:
- Range_of_Dates/Times:
- Beginning_Date:
- 20110601
- Ending_Date:
- 20110901
- Currentness_Reference:
- observed
- Status:
- Progress:
- Complete
- Maintenance_and_Update_Frequency:
- As needed
- Spatial_Domain:
- Description_of_Geographic_Extent:
- Water was collected via an intake pipe from a few hundred meters south of Dauphin Island in the northern Gulf of Mexico at high tide.
- Bounding_Coordinates:
- West_Bounding_Coordinate:
- -88.080239
- East_Bounding_Coordinate:
- -88.080239
- North_Bounding_Coordinate:
- 30.243423
- South_Bounding_Coordinate:
- 30.243423
- Keywords:
- Theme:
- Theme_Keyword_Thesaurus:
- None
- Theme_Keyword:
- oil
- Theme_Keyword:
- dispersant
- Theme_Keyword:
- dispersed oil
- Theme_Keyword:
- growth
- Theme_Keyword:
- production
- Theme_Keyword:
- grazing
- Theme_Keyword:
- viral lysis
- Theme_Keyword:
- microbial loop
- Theme_Keyword:
- phytoplankton
- Theme_Keyword:
- chlorophyll a
- Theme_Keyword:
- prokaryote
- Theme_Keyword:
- microzooplankton
- Theme_Keyword:
- virus
- Theme_Keyword:
- protist
- Theme_Keyword:
- heterotrophic nano-flagellate
- Theme:
- Theme_Keyword_Thesaurus:
- ISO 19115 Topic Category
- Theme_Keyword:
- biota
- Theme_Keyword:
- environment
- Theme_Keyword:
- inlandWaters
- Theme_Keyword:
- oceans
- Place:
- Place_Keyword_Thesaurus:
- None
- Place_Keyword:
- Dauphin Island Sea Lab
- Place_Keyword:
- DISL
- Access_Constraints:
- Permission to access these data must be given by Dr. Alice Ortmann of the Dauphin Island Sea Lab.
- Use_Constraints:
- Acknowledgment of the Dauphin Island Sea Lab (DISL) and GoMRI (Gulf of Mexico Research Initiative, Year 1 block grant to MESC) is required in products developed from these data, and such acknowledgment as is standard for citation and legal practices for data source is expected by users of these data. Users should be aware that comparison with other data sets for the same area from other time periods may be inaccurate due to inconsistencies resulting from changes in mapping conventions, data collection, and computer processes over time. The distributor shall not be liable for improper or incorrect use of these data, based on the description of appropriate/inappropriate uses described in the metadata document. These data are not legal documents and are not to be used as such.
- Point_of_Contact:
- Contact_Information:
- Contact_Organization_Primary:
- Contact_Organization:
- Dauphin Island Sea Lab
- Contact_Person:
- Alice Ortmann
- Contact_Position:
- principal investigator
- Contact_Address:
- Address_Type:
- mailing and physical
- Address:
- 101 Bienville Blvd.
- City:
- Dauphin Island
- State_or_Province:
- AL
- Postal_Code:
- 36528
- Country:
- USA
- Contact_Voice_Telephone:
- 251-861-2141 x7526
- Contact_Electronic_Mail_Address:
- aortmann@disl.org
- Cross_Reference:
- Citation_Information:
- Originator:
- Alice Ortmann
- Originator:
- Jennifer Anders
- Originator:
- Naomi Shelton
- Originator:
- Limin Gong
- Originator:
- Anthony G. Moss
- Originator:
- Robert H. Condon
- Publication_Date:
- 20120731
- Title:
- Dispersed Oil Disrupts Microbial Pathways in Pelagic Food Webs
- Geospatial_Data_Presentation_Form:
- journal article
- Series_Information:
- Series_Name:
- PLoS ONE
- Issue_Identification:
- 7(7): e42548. doi:10.1371/journal.pone.0042548
- Online_Linkage:
- http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0042548
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- Data_Quality_Information:
- Logical_Consistency_Report:
- not applicable
- Completeness_Report:
- The dataset has no omissions.
- Lineage:
- Methodology:
- Methodology_Type:
- Laboratory
- Methodology_Identifier:
- Methodology_Keyword_Thesaurus:
- None
- Methodology_Keyword:
- Mesocosms
- Methodology_Description:
- Replicated mesocosms (n=5) were exposed to different treatments (control, glucose, oil, dispersant and dispersed oil) and sampled over time for biomass of different microbial groups.
- Process_Step:
- Process_Description:
- Abundances: At specific times, 4.5 ml samples were collected from each mesocosm (from t=0 to t=5 days) and fixed in 0.5% EM grade glutaraldehyde and flash frozen in liquid nitrogen. Samples were stored at -80C until processing. Samples for viruses were diluted 100x in filtered TE and stained with Sybr Green I at 80C for 10 min. Samples were then run for 2 min on a FACSCalibur flow cytometer with calibrated flow rate to determine abundances. For prokaryotes, dilution was 10X with TE, and samples were stained for 10 min at room temperature before analysis on the flow cytometer. For heterotrophic nanoflagellates, 50 ml samples were fixed in 0.5% EM grade glutaraldehyde for 30 min before being filtered through a 0.8 micron black polycarbonate filter. Cells were stained with DAPI and counted using epifluorescence microscopy. For phytoplankton and ciliates, ~100 ml was fixed in acidic Lugol's solution and counted using an inverted microscope.
- Process_Date:
- Unknown
- Process_Step:
- Process_Description:
- Growth rates and virus production rates: At t=0, 1 and 3 days, sub-samples were collected from each mesocosm and 5 ml of water was added to 45 ml of 30 kDa filtered water. Sub-samples were collected at t=0, 3, and 6 hours and the abundance of prokaryotes and viruses determined. Growth and viral production rates were determined based on the regression of ln(cell or virus number) vs time over 6 hours.
- Process_Date:
- Unknown
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- Entity_and_Attribute_Information:
- Detailed_Description:
- Entity_Type:
- Entity_Type_Label:
- Abundances and rates measured in mesocosm experiments measuring the effects of oil, dispersant and dispersed oil on microbial communities.
- Entity_Type_Definition:
- An MS-Excel spreadsheet containing data from two mesocosm experiments.
- Entity_Type_Definition_Source:
- DISL: Ortmann Lab
- Attribute:
- Attribute_Label:
- Sample
- Attribute_Definition:
- Sample number.
- Attribute_Definition_Source:
- DISL
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 1
- Range_Domain_Maximum:
- 393
- Attribute_Units_of_Measure:
- count
- Attribute:
- Attribute_Label:
- Experiment
- Attribute_Definition:
- Experiment identifier.
- Attribute_Definition_Source:
- DISL
- Attribute_Domain_Values:
- Enumerated_Domain:
- Enumerated_Domain_Value:
- June
- Enumerated_Domain_Value_Definition:
- Experiment carried out June 2011
- Enumerated_Domain_Value_Definition_Source:
- DISL
- Enumerated_Domain_Value:
- August
- Enumerated_Domain_Value_Definition:
- Experiment carried out August 2011
- Enumerated_Domain_Value_Definition_Source:
- DISL
- Attribute:
- Attribute_Label:
- Time
- Attribute_Definition:
- Time sub-samples were collected relative to addition of treatments.
- Attribute_Definition_Source:
- DISL
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- -0.085
- Range_Domain_Maximum:
- 9.72
- Attribute_Units_of_Measure:
- days
- Attribute:
- Attribute_Label:
- Treatment
- Attribute_Definition:
- Treatment applied to the mesocosms.
- Attribute_Definition_Source:
- DISL: Ortmann Lab
- Attribute_Domain_Values:
- Enumerated_Domain:
- Enumerated_Domain_Value:
- Control
- Enumerated_Domain_Value_Definition:
- Control treatment
- Enumerated_Domain_Value_Definition_Source:
- DISL
- Enumerated_Domain_Value:
- Dispersant
- Enumerated_Domain_Value_Definition:
- Treatment with dispersant added
- Enumerated_Domain_Value_Definition_Source:
- DISL
- Enumerated_Domain_Value:
- Glucose
- Enumerated_Domain_Value_Definition:
- Treatment with glucose added
- Enumerated_Domain_Value_Definition_Source:
- DISL
- Enumerated_Domain_Value:
- Oil
- Enumerated_Domain_Value_Definition:
- Treatment with oil added
- Enumerated_Domain_Value_Definition_Source:
- DISL
- Enumerated_Domain_Value:
- Oil+Dispersant
- Enumerated_Domain_Value_Definition:
- Treatment with oil and dispersant added
- Enumerated_Domain_Value_Definition_Source:
- DISL
- Attribute:
- Attribute_Label:
- Replicate
- Attribute_Definition:
- Letter representing one of 5 replicates of each treatment.
- Attribute_Definition_Source:
- DISL: Ortmann Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- A
- Range_Domain_Maximum:
- E
- Attribute:
- Attribute_Label:
- ProkaryotesCells/ml
- Attribute_Definition:
- Abundance of prokaryotes cells measured with flow cytometry.
- Attribute_Definition_Source:
- DISL: Ortmann Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 781588.45
- Range_Domain_Maximum:
- 14354060.89
- Attribute_Units_of_Measure:
- cells per milliliter
- Attribute:
- Attribute_Label:
- Viruses/ml
- Attribute_Definition:
- Abundance of viruses measured with flow cytometry.
- Attribute_Definition_Source:
- DISL: Ortmann Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 16348375.45
- Range_Domain_Maximum:
- 244653488.78
- Attribute_Units_of_Measure:
- viruses per milliliter
- Attribute:
- Attribute_Label:
- Dinoflagellates/l
- Attribute_Definition:
- Abundance of dinoflagellates counted on an inverted microscope.
- Attribute_Definition_Source:
- DISL: Ortmann Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 10606.06
- Range_Domain_Maximum:
- 356363.64
- Attribute_Units_of_Measure:
- cells per liter
- Attribute:
- Attribute_Label:
- Diatoms/l
- Attribute_Definition:
- Abundance of diatoms counted on an inverted microscope.
- Attribute_Definition_Source:
- DISL: Ortmann Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 2121.21
- Range_Domain_Maximum:
- 890909.09
- Attribute_Units_of_Measure:
- cells per liter
- Attribute:
- Attribute_Label:
- Ciliates/l
- Attribute_Definition:
- Abundance of ciliates counted on an inverted microscope.
- Attribute_Definition_Source:
- DISL: Ortmann Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 0
- Range_Domain_Maximum:
- 458181.82
- Attribute_Units_of_Measure:
- cells per liter
- Attribute:
- Attribute_Label:
- HNF/ml
- Attribute_Definition:
- Abundance of heterotrophic nanoflagellates counted on using epifluorescence microscopy.
- Attribute_Definition_Source:
- DISL: Ortmann Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- 0
- Range_Domain_Maximum:
- 292000
- Attribute_Units_of_Measure:
- cells per milliliter
- Attribute:
- Attribute_Label:
- Prokaryote Growth h-1
- Attribute_Definition:
- Growth rate of prokaryotes.
- Attribute_Definition_Source:
- DISL: Ortmann Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- -0.03
- Range_Domain_Maximum:
- 0.39
- Attribute_Units_of_Measure:
- per hour
- Attribute:
- Attribute_Label:
- Virus Production h-1
- Attribute_Definition:
- Production rate of viruses.
- Attribute_Definition_Source:
- DISL: Ortmann Lab
- Attribute_Domain_Values:
- Range_Domain:
- Range_Domain_Minimum:
- -0.75
- Range_Domain_Maximum:
- 0.71
- Attribute_Units_of_Measure:
- per hour
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- Metadata_Reference_Information:
- Metadata_Date:
- 20140519
- Metadata_Contact:
- Contact_Information:
- Contact_Organization_Primary:
- Contact_Organization:
- Dauphin Island Sea Lab
- Contact_Position:
- Data Management Specialist
- Contact_Address:
- Address_Type:
- mailing and physical
- Address:
- 101 Bienville Blvd.
- City:
- Dauphin Island
- State_or_Province:
- Al
- Postal_Code:
- 36528
- Country:
- USA
- Contact_Voice_Telephone:
- 251-861-2141
- Contact_Electronic_Mail_Address:
- metadata@disl.org
- Hours_of_Service:
- 8-5:00 CST
- Contact_Instructions:
- Please email the metadata specialist for further information.
- Metadata_Standard_Name:
- FGDC Content Standard for Digital Geospatial Metadata
- Metadata_Standard_Version:
- FGDC-STD-001.1-1999
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